Gene cloning and DNA analysis : an introduction / T.A. Brown.

Includes bibliographical references and index.

Part I The basic principles of gene cloning and DNA analysis -- Why gene cloning and DNA analysis are important -- Vectors for gene cloning : plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for E. coli -- Cloning vectors for Eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- Part II The applications of gene cloning and DNA analysis in research -- Sequencing genes and genomes -- Studying gene expression and function -- Studying genomes -- Part III The applications of gene cloning and DNA analysis in biotechnology -- Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology.